Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQQQVKNEFPTLEDIHAAAERLDGLVVKTPFVFSETISKTLGAEMWLKFENLQFTASFKERGALNKLLSLSEQEKQHGVIAASAGNHAQGVAYHAQRTGVTATIVMPKSTPNVKVQRVREYGARVILHGQDFSEAAAEMHRVAQEESLTIIHPFDDAEIIAGQGTIALEMLEAVPDLDILVVPIGGGGLISGIAIAAKSINPKIKIIGVQSVVYPSMAKLLCNYQLAVSMGS--------TVAEGIAVKTPGELTTQIAKHFVDDIVVVTEDMIEEAIALLLNIEKTVCEGAGATGIAAIMSRSDLFLGHKVGVVLSGGNIDTRVMVSVLQRHLTRTGRMVRICVELSDNPGALARLTAIIAEQGGNIYELRHERFAATSRAKESAVSVDIELKSAPDLEPLIQAMQLEGYIVRKEEI 2ZPU Chain:A ((8-313)) ----------PTYDDVASASERIKKFANKTPVLTSSTVNKEFVAEVFFKCENFQKMGAFKFRGALNALSQLNEAQRKAGVLTFSSGNHAQAIALSAKILGIPAKIIMPLDAPEAKVAATKGYGGQVIMYDRYKDDREKMAKEISEREGLTIIPPYDHPHVLAGQGTAAKELFEEVGPLDALFVCLGGGGLLSGSALAARHFAPNCEVYGVE----PEAGN---DGQQSFRKGSIVHIDTPKTIADGAQTQHLGNYTFSIIKEKVDDILTVSDEELIDCLKFYAARMKIVVEPTGCLSFAAARAMKEKLKNKRIGIIISGGNVD------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZPU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1973 -102437 -51.92 -343.75 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -51.92 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_2ZPU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZPU-query.scw PDB file : Tito_Scwrl_2ZPU.pdb: