Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKPKGKLFGDRGYVGTNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLRKRGIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 4A34 Chain:I ((23-39)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GDEIVLADANYPSASCA------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4A34.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 13 -1516 -116.62 -89.18 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain I : 0.48 3D Compatibility (PKB) : -116.62 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_4A34.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A34-query.scw PDB file : Tito_Scwrl_4A34.pdb: