Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLKVCTLLPCSLVIAACSVNSSSTPPTLSSSTPQVQKSSIEISCQDLQNPAYRQAVLNAINQIRQDSRQCGQQYFSAAKPLSWNNNLYQGANAHSKDMANNNFLGHVGSTGLDLRARLKKYHMLSKANGENVASGQKTLNEVLAKWIA--------------SPLHCSNIMNPRYTEYAIACASDQSAKQRSYWTQQFAGF 5JYS Chain:A ((158-282)) --------------------------------------------------LSDFASSVLAEHNKKRALH--------KDTPALSWSDTLASYAQDYADNYDCSGTLTHSG-----------------GPYGENLALGYD-GPAAVDAWYNEISNYDFSNPGFSSNTGHFTQVVWKSTTQVGCGIKTCGG-AWGDYVICSYDP-

General information: TITO was launched using: RESULT: Template: 5JYS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 400 -13158 -32.90 -118.54 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -32.90 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.251

(partial model without unconserved sides chains): PDB file : Tito_5JYS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JYS-query.scw PDB file : Tito_Scwrl_5JYS.pdb: