Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVVAFKQVDVFTSQAFKGNPVAVIMDASTLTSEQMQAIANWTNLSETTFVLPAIDSQADYQVRIFTPQSELPFAGHPTIGTAYALLEAGLVKAKEGQLVQQCAAGLVTLTVNDSNH----ISFELPKPKITPLDALQTQKLAEILKCK--INTQWNAALVDVGARWVVLQAVNAKAVLASQPDLNALKQFSLEMKVTGSTIYGFYEESNEQKRIEVRSFAPAYGVNEDPVCGSGNGSVASFIRYHSILPAQNDVMLSSQGQALGR--------EGQLQLELHQDKILVGGAAVTCIDGTIKL 1U1V Chain:A ((27-298)) --------IDAFASVPLEGNPVAVFFDADDLPPAQMQRIAREMNLSESTFVLKPRNG-GDALIRIFTPVNELPFAGHPLLGTAIAL----GAHTDNHRLYLETQMGTIAFELERQNGSVIAASMDQPIPTWTALG-----RDAELLKALGISDSTFPIEIYHNGPRHVFVGLPSIDALSALHPDHRALSNFH------DMAINCF---AGAGRRWRSRMFSPAYGVVEDAATGSAAGPLAIHLARHGQIEFGQPVEI-LQGVEIGRPSLMFAKAEGRAE---QLTRVEVSGNGVTFGRGTIVL

General information: TITO was launched using: RESULT: Template: 1U1V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1537 -63134 -41.08 -244.70 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -41.08 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_1U1V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U1V-query.scw PDB file : Tito_Scwrl_1U1V.pdb: