Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPLYVTSGEPAGIGPDICLSLAKRVDERPVVVLADRNLLHQRAQKLGLDIKFLEYSGQAESSLQGELYIEHVPLETIVVDGQLNAANAAYVLEQLRRSADYAMSGKSVGVATAPVQKSVINDAGILFSGHTEYYQEFAGVERVVMMLATKTLRVALATTHLPLRDVADAITKERLHQVIDILLRDL--KTKFKINDPRVLVCGLNPHAGEDGYLGREE-------IDTINPVLES-----------------------YRSQGVKLSLSLPADTLFTPEHLKNADAVLAMYHDQGLPVLKSQGFGEAINITLGLPFIRTSVDHGTALSLA-GTGLAKSSSLNVAADLALSLAAS 3TSN Chain:A ((102-363)) ---------------------------------------------------------------------------------GEIDAKSGLYGFLSFKAASYFVYEKHAHALLTLPIHKKAWEDAGLKYKGHTDALRDFFK-KNAIMMLGCKELFVGLFSEHIPLAKVSKKITFKNL----SIFLKDFYKETHFK----KMGLLGFNPHAGDYGVIGGEEEKIMEKAIAFVNAFLHSKKDEKFFKKALKDENLQKELLLNFKGKGVYLPYPLVADTAFTKTGLKNCNRLVAMYHDLALAPLKALYFDKSINVSLNLPIIRVSVDHGTAFDKAYKNAKINTKSYFEAAKFAINL---

General information: TITO was launched using: RESULT: Template: 3TSN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 -67466 -68.08 -294.61 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -68.08 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_3TSN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TSN-query.scw PDB file : Tito_Scwrl_3TSN.pdb: