Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QVFGCFVQMSVRTQDSKDETNISSGGYLLTTNPGQLVFTDG-----------------NNNITVKVDKDCQSAGISYHRSEVHFTALQKLSENGGPFEFDQPLIELQ 2QTV Chain:A ((352-456)) ---GCYDQIGM--SEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLFAKDEEGYLKMAFNGNMAVKTSKDLKVQGLIGHASAVKKTDANNISES--------------

General information: TITO was launched using: RESULT: Template: 2QTV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 101 -2131 -21.09 -30.01 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -21.09 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.074

(partial model without unconserved sides chains): PDB file : Tito_2QTV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QTV-query.scw PDB file : Tito_Scwrl_2QTV.pdb: