Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QCNHHLLYNGRHWGTIRKKAGWAVRFYEEKPGQPKRLVTICKNASPVHCNYLKCTNLA--------AGFSAG--TSTDVLSSGTVGSIGNDPQAQRQ 3TY2 Chain:B ((74-119)) -----------------------------------------VEGTPTDCVHLAITGVLPEMPDMVVAGINAGPNLGDDVWYSGTVAA----------

General information: TITO was launched using: RESULT: Template: 3TY2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 36 -1956 -54.33 -54.33 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -54.33 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.196

(partial model without unconserved sides chains): PDB file : Tito_3TY2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TY2-query.scw PDB file : Tito_Scwrl_3TY2.pdb: