Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF 4GAL Chain:A ((1-135)) -SNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF

General information: TITO was launched using: RESULT: Template: 4GAL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 689 -73268 -106.34 -542.73 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -106.34 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.667

(partial model without unconserved sides chains): PDB file : Tito_4GAL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GAL-query.scw PDB file : Tito_Scwrl_4GAL.pdb: