Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLP---GVCKVSTDLLREGA-PIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADAR---------------------QEEDSYEIFICHANVIRYIVCRALQFPPE----GWL----RLSLNNGSITHLVIRPNG--------RVALRTLGDTGFMPPDKITRS 3E9C Chain:A ((2-250)) -----------------------------------------------------------------------------------------------LTFALTIVRHGET--------DTPLSDTGHQQAAAAGRYLKD--LHFTNVFVSNLQRAIQTAEIILGNNLHSSATEMILDPLLRERGF--------------GETLEQVKTRFKMFLKSLFQRMFEEHGSALSSADQPVIAGLADDGAQNVPVHALMVSHGAFIRISVRHLVEDLQCCLPAGLKMNQVFSPCPNTGISRFIFTIHREESVLRATRIQGVFINRKDHL--------

General information: TITO was launched using: RESULT: Template: 3E9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 687 -45713 -66.54 -282.18 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -66.54 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3E9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E9C-query.scw PDB file : Tito_Scwrl_3E9C.pdb: