Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVH--TNAENKLYLAENYCFDSIPKLIHYH 1LUN Chain:A ((8-91)) WYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYH

General information: TITO was launched using: RESULT: Template: 1LUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 346 -19447 -56.21 -237.16 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -56.21 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_1LUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LUN-query.scw PDB file : Tito_Scwrl_1LUN.pdb: