Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKFSMEKENRGKNMAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKLVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYKIKFISCDRDH 4ZSB Chain:A ((28-160)) -------------------------------------------------------------------------------------------QLLDIGYITADDRLAGLLDITAGGRVL--------------RIERLRMANGEPMAIETTHLSAKRFPALRRSLVKYTSLYTALAEVYDVHLAEA--EETIETSLATPREAG-LLGTDVGLPMLMLSRHSQ-DRTGQPVEWVRSVYRGDRYK----------

General information: TITO was launched using: RESULT: Template: 4ZSB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 601 -47789 -79.52 -359.32 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -79.52 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_4ZSB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZSB-query.scw PDB file : Tito_Scwrl_4ZSB.pdb: