Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKSKMTNSNYKLTKEDFNQINKRRLFTFQLGWNYERMQASGYLYMILPQLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKTGLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEFAYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQDILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM 2JTT Chain:C ((192-212)) ----------------------------------------------LMNVLKKIYEDGDDDMKRTIN-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2JTT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 5 64 12.80 3.05 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain C : 0.44 3D Compatibility (PKB) : 12.80 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.711

(partial model without unconserved sides chains): PDB file : Tito_2JTT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JTT-query.scw PDB file : Tito_Scwrl_2JTT.pdb: