Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVWYNLFLKKEREVISMRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVYDSRTEKLTFGKEVENLEITLHQHTLTITDSF-DDQIHISYHPSLSAHHDLITNQNDRTLSLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTII-NASLENATLNTN-SYILRIEG-SRIKNSKLT--TPNIVNIFDTVLTDSQLES-TENHFHAE-NIQVHGKVELTA-K-DYLRIILDQKESQRINWDIS-SNYGSIFQFTREKPESRGTELSNPYKTEKTDVKDQLIARSDDNIDLISTPSRR 4OPW Chain:B ((12-205)) ---------------------------------------------------------------STKKITI-DDFNAIKFDG-VIDFNYEQSESTPHIEITVD--ENLHPYVNIDIQDRVLTVGFKGAK---V-D-------------HFTKFIVKTNSK---WLKEVKAS-GNANFIANSPLKGDELKINANSNCLVQLKQKVEVGKLDLNVSGSANMVVNELKTDKLECSINGSGTINLKAGN--AEEADYSITTDGEIMAFGVAV--P--EVNCKITGKGSAQIHP-------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1056 65341 61.88 357.05 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : 61.88 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_4OPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPW-query.scw PDB file : Tito_Scwrl_4OPW.pdb: