Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFMQTKLLQVQNDLTKINAQIEEKKTELDDAKQ-EVNEL----------LRAERLKEIANSHDL-QLNNENIRIAE 3FFZ Chain:A ((686-746)) --------------------------------------------------VSNWMTKINTQFNKRKEQMYQALQNQVNAIKTIIESKYNSYTLEEKNELTNKYDIKQIENE------

General information: TITO was launched using: RESULT: Template: 3FFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 15 708 47.17 14.44 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 47.17 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_3FFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FFZ-query.scw PDB file : Tito_Scwrl_3FFZ.pdb: