Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------MRKVWHKLLPLLPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN------------------------------- 2JX0 Chain:A ((640-770)) LDGDPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

General information: TITO was launched using: RESULT: Template: 2JX0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 71 -2283 -32.15 -43.08 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -32.15 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_2JX0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JX0-query.scw PDB file : Tito_Scwrl_2JX0.pdb: