Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKNIYFVVLDLHT-------TDRDKIIQLFKDWTDYSAKLVEGELVKKDGQNALFPPSDTGE---TVGLNPHRLTLTFGVSASFLKRMNLENKRPRLFRDLPLFPKEQLREKYTGGDIVIHACADDEQIAFHAIRNLIRKGRNAVPLRWSQSGF----AAIGDRMETPWNLFGFKDGTANPTKEQD-FDRVIWADSKD-----WMENGSYMAVRRIQMFLETWDRTSLEEQENTFGRYKESGAPFGKKNEFDEVDLSLLPDDSHVCLAK---EVDKPLLRRSYSYSDGIDEKTGQFDTGLLFISFQKDP-DNFVKVQTNLG-ATDKMNEYITHIGSGLFTCFGGVEKGGYIGQKLLEG 5FW4 Chain:A ((82-430)) ----VHVIALDLAEEARKNPDTARDSAAAALRSWTELAARLHEES------------PHDIAEGAASAGLLPASLMVTVGIGGSLLSAIDAEDRRPDALADLPEFSTDDLHPRWCGGDFMLQVGAEDPMVLTAAVEELVAAAADATAVRWSLRGFRRTAAAARDPDATPRNLMGQIDGTANPAQDHPLFDRTITARPADNPAHAWMDGGSYLVVRRIRMLLTEWRKLDVAARERVIGRRLDTGAPLGSRNET------LIPENAHVRLASPENNLGARMFRRGYSYDQGWRDD-GVRDAGLLFMAWQGDPATGFIPVQRSLADQGDALNRYIRHEGSALFAVPAARE-GRYLGQDLIEG

General information: TITO was launched using: RESULT: Template: 5FW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1498 46212 30.85 149.07 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 30.85 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_5FW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FW4-query.scw PDB file : Tito_Scwrl_5FW4.pdb: