Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKLLRVDTKVIM-GSFFLVLSSLLALLLPLILKGLIDGSSIENIGS----KVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKS 3QF4 Chain:B ((31-130)) ----YLRPHTFTLIMVFVFVTVSSILGVLSPYLIGKTIDVVFVPRRFDLLPRYMLILGTIYALTSLLFWLQGKIMLTLSQDVVFRLRKELFEKLQRVPVGFFDR-

General information: TITO was launched using: RESULT: Template: 3QF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 155 -23584 -152.15 -248.25 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -152.15 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_3QF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QF4-query.scw PDB file : Tito_Scwrl_3QF4.pdb: