Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKLIEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD 3FB9 Chain:B ((1-88)) MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLE------KNRLGKLIEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD

General information: TITO was launched using: RESULT: Template: 3FB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 295 -27676 -93.82 -337.51 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.99 3D Compatibility (PKB) : -93.82 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.636

(partial model without unconserved sides chains): PDB file : Tito_3FB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FB9-query.scw PDB file : Tito_Scwrl_3FB9.pdb: