Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLQFIASCPQHGVLDHPPNECHITQEYVAALLFKQLGNYGFDA-QHIVHEHE----KVEVSIDNHLL-PLSITCQQTDHDGHLMCEISANPDEEQDWFEKIETQSIIRQLAQAVENSLKADHSFSAFEWKS 2E3R Chain:A ((28-119)) -------------------------VEEMVQNHMTYSLQDV----WQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLA--------------------

General information: TITO was launched using: RESULT: Template: 2E3R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 125 7654 61.23 98.13 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 61.23 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_2E3R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E3R-query.scw PDB file : Tito_Scwrl_2E3R.pdb: