Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPEGEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDE--GHGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVG--L----------------R--------H--------YKT----PEIQGDILLIHGEQDDITLLSDAIEWAKP-QKHPITILSGANHFFTGYL-KQLRQIITRFIIMK 3PFB Chain:A ((18-265)) ----YFQGMATITLERDGLQLVGTREEPFGEIYDMAIIFHGFTANRN---TSLLREIANSLRDENIASVRFDFNGHGDSDGKFENMTVLNEIEDANAILNYVKTDPHVRNIYLVGHAQGGVVASMLAGLYPD--LIKKVVLLAPAATLKGDALEGNTQGVTYNPDHIPDRLPFKDLTLGGFYLRIAQQLPIYEVSAQFTKPVCLIHGTDDTVVSPNASKKYDQIYQNSTLHLIEGADHCFSDSYQKNAVNLTTDFLQ--

General information: TITO was launched using: RESULT: Template: 3PFB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1137 -32038 -28.18 -155.52 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -28.18 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3PFB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFB-query.scw PDB file : Tito_Scwrl_3PFB.pdb: