Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMLSLSVFFKGLGIGSGLIVAIGAQNAFVLKQGLKQ-QYVFWLCLICALSDSILIACGVLGFAEIMTASPILITVAKYLGATFLFVYGAKAFYAAFKTNQGMELDSSQKQT-LTQALITCLAFTWLNPHVYLDTIVLIGSVATQLEDKISFALGSILASWIFFFSLGYGARFLKPLFINPKAWKILDFMIGCVMWSIAISLLI--- 5JBL Chain:A ((21-191)) ----NEPQLLIETWGQPGEIID----GVPMLESGLKPGLYIEGIFLQAEVVNRNK---------RLYPKRILEKAVKDYINEQVLT-----------KQALGELNAPPRANVDPMQAAIIIEDMWWKGNDVYGRARVIEGD-----HGPGDKLAANIRAGWIP----GVASRGLGSLTDTNEGYRIVNEG-----FKLTVGVDAVWG

General information: TITO was launched using: RESULT: Template: 5JBL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 795 -73247 -92.13 -457.79 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -92.13 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.073

(partial model without unconserved sides chains): PDB file : Tito_5JBL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JBL-query.scw PDB file : Tito_Scwrl_5JBL.pdb: