Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILITGANTGIGFATAEQLVKQGQHVILACRNPQKAQEAQNKLRSLDQGQVDVASLDLNSLELTQKAAEEIADKYGSLDVLINNAGLFSK--TKQLTVDGFEQKFGVNYLGHFLLTQKLLPVLKQFPQAHIIHLASIAHWVGSIKPNKFRAEGFYNPLFYYGQSKLANLLFSNALAEQLADSSITNNALHPG----GVASDIYRDLPKPVYAAMKVGLVPTSVPAKLITEMATGDTWQNRNGEYVSAHMPDWKSSHAKNQQLARDLYQQSMNLVEKFL 1VL8 Chain:A ((25-238)) ---LVTGGSRGLGFGIAQGLAEAGCSVVVASRNLEEASEAAQKLTEKYGVETMAFRCDVSNYEEVKKLLEAVKEKFGKLDTVVNAAGINRRHPAEEFPLDEFRQVIEVNLFGTYYVCREAFSLLRESDNPSIINIGSLTVEEVTM-PN----------ISAYAASKGGVASLTKALAKEWGRYGIRVNVIAPGWYRTKMTEAVFSDPEKLDYMLKRIPLGRTGVPEDL---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1045 -46439 -44.44 -223.26 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -44.44 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1VL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VL8-query.scw PDB file : Tito_Scwrl_1VL8.pdb: