Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MKISLSSFQIGSFFALCSAFLFSTKAIFIKQ------TYALSPLVDGTVLMALRM-------LSALPFFLLICWFNRHHNQGIKKKDWLILI----------FAGLL-----------GYYFASWLDFMSLMFIS-------------ASLERIILFLYPTLTVIAS--------SLLYKQKLDVKSLFAIFLSYGGTVLVMLQEHNNATIQGKFWLGTSFV------FASALAFAGYLLLTPPLIKKFGSWNFTGLALTVACIGTLT----------------------HYTLSTSHPFQLLLQLPSSVIWYGIGLGFLVTVLPTVMLMQSIERLGASQ---------------SAMIASIGP------------------------VLTILLAVAFLNEHLNIWQWVGCFL----NIIGVMMITLRKKKLKH--------- 5L3T Chain:B ((1-455)) MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTRFKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVNKLNNIYFRIESPQLCSNIFKN-FQPKSMLAHFNEYQ-----LDQQIEYRYLLGRYYLLNSQVHNAFVQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQNKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLR--ANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW

General information: TITO was launched using: RESULT: Template: 5L3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1250 -92238 -73.79 -307.46 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -73.79 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_5L3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3T-query.scw PDB file : Tito_Scwrl_5L3T.pdb: