Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHWLEFMGNHPILFGTLGVLIVLFFIFEGQRNGRKISPQSLGILVKAKNALLIDLRDSKDFREGHISGSRNIPYSQIASHADELKASDRPLVF-ICNLGQVAGSALQKVAH----HDSYRLDGGISNWKAQGLPLVKSKPKA 5HPA Chain:A ((22-124)) ----------------------------------TTISPHDAQELI-ARGAKLIDIRDADEYLREHIPEADLAPLSVLEQSGLPAKLRHEQIIFH--QAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLPVAVN----

General information: TITO was launched using: RESULT: Template: 5HPA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 442 -13907 -31.46 -143.37 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.46 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_5HPA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HPA-query.scw PDB file : Tito_Scwrl_5HPA.pdb: