Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLITSDCINCDMCLPECPNTAIFEGNKVYEIDPLRCTECVGFYDAPTCKAVCPIDCIKQDPAHIENKEQLLEKFKGLNLIGSFSS 2FDN Chain:A ((1-50)) -AYVINEACISCGACEPECPVNAISSGDDRYVIDADTCIDC------GACAGVCPVD------------------------------

General information: TITO was launched using: RESULT: Template: 2FDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 165 -42107 -255.19 -842.14 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -255.19 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_2FDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FDN-query.scw PDB file : Tito_Scwrl_2FDN.pdb: