Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKLNALLCSAVLATSMVTVAHADNTTHVAAASALGSIAGTAIGKSMGGTNGGYIGAALGAAGGSALGNKISKDRDAD--------RSSKYWKKNTVIIVTNYRDEIGLKTPSGVFL 5EYU Chain:A ((225-286)) ----------------------------------------------------GTINLILGA--GSEVGDVMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIF-

General information: TITO was launched using: RESULT: Template: 5EYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 158 7644 48.38 141.55 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 48.38 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_5EYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EYU-query.scw PDB file : Tito_Scwrl_5EYU.pdb: