Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNHDLIHSTAIIDPSAVIASDVQIGPYCIIGPQVTIGAGTKLHSHVVVGGFTRIGQNNEIFQFASVGEVCQDLKYKGEETWLEIGNNNLIREHCSLHRGTVQDNALTKIGSHNLLMVNTHIAHDCIVGDHNIFANNVGVAGHVHIGDHVIVGGNSGIHQFCKIDSYSMIGGASLILKDVPAYVMASGNPAHAFGINIEGMRRKGWSKNTIQGLREAYKLIFKSGLTSVQAIDQIKSEI---LPSVPEAQLLIDSLEQSERGIVR 2JF2 Chain:A ((9-264)) -----FVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVLKSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGTVQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVHQFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNAYKLIYRSGKT----LDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR

General information: TITO was launched using: RESULT: Template: 2JF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1551 -209912 -135.34 -829.69 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -135.34 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_2JF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JF2-query.scw PDB file : Tito_Scwrl_2JF2.pdb: