Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLPILSFPDPRLRTIAKPVEEVTDEIRQLAADMFETMYAAPGIGLAASQVDRHIQLIVMDLSESKDEPMVFINPKVTPLTEETQPYEEGCLSVPQIYDKVDRPSRVKIEAINLEGQAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRVREKVEKIVRQREREKVAVKR 2W3U Chain:A ((1-165)) -SVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGET-GIEEGCLSIPEQRALVPRAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVEKLDRLK---------

General information: TITO was launched using: RESULT: Template: 2W3U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 809 -91769 -113.43 -556.17 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -113.43 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.667

(partial model without unconserved sides chains): PDB file : Tito_2W3U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W3U-query.scw PDB file : Tito_Scwrl_2W3U.pdb: