Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREIILISFLGPDQPNQFTRLMQVLSVHSLQILDVGQAVIHNQLTLGIVVASENETATALAMKEILILAHDIGLTVRFK--PISGAEYDQWVSEGGRTRYIVTALAPELTAAHLQAVTQIVSSQGFNIETVTRLSGR--VDLEKDSTLPRRACVQFGLSSGPTLDAQAMRAACLLLSSELNIDVAVQEDNAYRRNRRLVCFDMDSTLIEQEVIDELALEAGVGEQVAEITERAMQGELDFQQSFRARVALLKGLDASVLPKIAERLTITEGAERLISTLKVLGYKTAILSGGFQYFAEYLQAKLGIDEVHANVLDVQDGVVTGEVKGVIVDGARKAELLRELANKLGISLEQAMAVGDGANDLPMLAIAGLGVAYRAKPLVRQNANQAISSVGLDGVLYLLGMHDKDLSRA 5IT0 Chain:A ((5-384)) ----VLITVTGVDQPGVTATLFEVLSRHGVELLNVEQVVIRHRLTLGVLVCCPADVADGPALR------HDVEAAIRKVGLDVSIERSDDVPIIREPSTHTIFVLGRPITAAAFGAVAREVAALGVNIDLIRGVSDYPVIGLELRVSVP------------PGADG-ALRTALNRVSSEEHVDVAVEDYTLERRAKRLIVFDVDSTLVQGEVIEMLAAKAGAEGQVAAITDAAMRGELDFAQSLQQRVATLAGLPATVIDEVAGQLELMPGARTTLRTLRRLGYACGVVSGGFRRIIEPLAEELMLDYVAANELEIVDGTLTGRVVGPIIDRAGKATALREFAQRAGVPMAQTVAVGNGANNIDMLAAAGLGIAFNAKPALREVADASLSHPYLDTVLFLLGV--------

General information: TITO was launched using: RESULT: Template: 5IT0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2039 -95293 -46.73 -253.44 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -46.73 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_5IT0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IT0-query.scw PDB file : Tito_Scwrl_5IT0.pdb: