Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTQPMIEKLIEAHLDFLDEQFAQTQVIQQEFEQFYHWLGSRQLQHLWTFE--QVQQLIQKQILDTPA-SDFLIEQI--AEHIRFALIHPTNDTATVEDVIPVLTIDRIAQYVASKEEHRKKLIKTIVNNPAFSALLTQLIQQTMHDYLDESMTKRVPGVGRFMKMGKSVLETVTDSNLDDTINHYLQKNILKLSQMSKRVLNQHFDNDKLYHFQANVWHKVKTSPLSVLKNYIEVQDLTRTVGLGHEIWDHIRQTDYLKQQVHDGIYTWYVRNQERNFDLLLRDLNIDENLVKHELTELLVPVLQQLVTTGHLRRRARVYLEKFYYSEKALEILNNKGA 3M7V Chain:A ((106-257)) ---------------------------ITEPFDTFWNGFPEEIISKIEKFSGRKVIREANKPYSGTAVIDDFGPRQMETGELIIYTSADPVLQIAAHEDVIPLDELYRICEYARSITLERPALLGRIIARPYVGKPRNFTRTANRHDY---ALSPFAPTV--LNKLADAGVSTYAVGKINDIFN------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3M7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 617 22743 36.86 154.71 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 36.86 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_3M7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M7V-query.scw PDB file : Tito_Scwrl_3M7V.pdb: