Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTLDEMTTVTDDQSELTMSVLMTPDMANFSGNVHGGTILKLLDQVAYACASRYSGSYVVTLSVDKVNFKEPIYVGELVTFLASVNHVGRTSMEVGIRVEAQNIQKRTVRHTNSCYFTMVAVDEHGKPRKVPKLNLDTEWKRCRFEAAEHRKVLRLQENHNPSCSMYKKTS 1VPM Chain:A ((20-159)) ------------ERSRTIQTRLVLPPDTNHLGTIFGGKVLAYIDEIAALTAMKHANSAVVTASIDSVDFKSSATVGDALELEGFVTHTGRTSMEVYVRVHSNNLLTGERTLTTESFLTMVAVDESGKPKPVPQVEPQTEEEKRLYETAPARK-------------------

General information: TITO was launched using: RESULT: Template: 1VPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 684 -65362 -95.56 -466.87 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -95.56 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1VPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VPM-query.scw PDB file : Tito_Scwrl_1VPM.pdb: