Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNLTLSQFLQQE--KGNLTPELAQVIDTIAATCKTIDQALQKGALAGILGSAGNENVQGETQKKLDVISNDYLIDALKVHPHVGGLASEELDD--FTPAQENGEYLVLFDPLDGSSNIDINMCVGTIFSILPAKNAVTQA--QDFMQAGTQQVAAGYVLYGPSTMMALTVGNGVAFFTLDPETQTFLLTTENVQVSADTQEFAINASNQRHWEQPVKQYIEELLAGKTSVREKDFNMRWVACMVGDVHRILCRGGIFLYPYDLKDPKKAGRLRLMYEANPMSMLIEQAGGASTTGRVRILEIEPTELHQRVPVIIGSKNEVERVTSYH 2F3B Chain:A ((11-325)) --DVTLTRFVMEEGRKARGTGEMTQLLNSLCTAVKAISTAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVINVLKSSFATCVLVSEEDKNAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSIGTIFGIY-RKNSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMVNGVNCFMLDPAIGEFILVDRDVKIKKKGSIYSINEGYAKEFDPAITEYIQRKKFPPDN--SAPYGARYVGSMVADVHRTLVYGGIFMYPANKKSPK--GKLRLLYECNPMAYVMEKAGGLATTGKEAVLDIVPTDIHQRAPIILGSPEDV-------

General information: TITO was launched using: RESULT: Template: 2F3B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1789 50012 27.96 161.85 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 27.96 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_2F3B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F3B-query.scw PDB file : Tito_Scwrl_2F3B.pdb: