Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLSTIIEQAFEDCANFTAADCPSEIRQAVEEAIAGLDNGTLRVAEK-INGEWVVHQWLKKAVLLSFKLNDNKPIESC--DLRFYDKVETKFSGWTEEQFKAAGVRVVPPAVARRGSFQAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVPG-----NLPSADGKYSLYAAIIVKKVDAQTRAKTSLNDLLRAD 3EG4 Chain:A ((6-283)) LASLEKTIEKAFDERDGINTA-TRGEVREAVEQSLILLDRGEVRVAEKQADGNWHVNQWLKKAVLLSFRLNPMEVIKGGPGQSSWWDKVPSKFDGWTANEFEKAGFRAVPNCIVRHSAYIAPNAILMPSFVNLGAYVDKGAMIDTWATVGSCAQIGKNVHLSGGVGIGGVLEPMQAGPTIIEDNCFIGARSEVVEGCIVREGSVLGMGVFIGKSTKIVDRATGEVFYGEVPPYSVVVAGTMPGKNVPGENWGPSLYCAVIVKRADEKTRSKTSINELLR--

General information: TITO was launched using: RESULT: Template: 3EG4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1454 -27267 -18.75 -100.99 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -18.75 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_3EG4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EG4-query.scw PDB file : Tito_Scwrl_3EG4.pdb: