Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSARHSRLIILGSGPAGYSAAVYAARANLKPTLIAGLQLGGQLTTTTEVDNWPGDPEGLTGPVLMDRMQAHAERFGTELVYDHINEVDLNVRPFVLKGDMDEYTCDALIIATGATAQYLGLESEQKFMGQGVSACATCDGFFYKNQNVMVVGGGNTAVEEALYLSNIAEHVTLVHRRDSLRSEKILQDHLFAKEKESKISIVWNHEVEEVLGDNTGVTGVRLKSTKDDSKQEVQVQGLFIAIGHKPNTSMFEGQLNLRDGYIQVQSGTSGNATATSVAGVFAAGDVADSIYRQAITSAGSGCMAALDAEKYLDNL 5U63 Chain:A ((8-317)) ---KHAKLLILGSGPAGYTAAIYAARANLKPVLVTGLQQGGQLTTTDEIENWPGDFEMTTGSGLMQRMLQHAEKFETEIVFDHINRVDLSSRPFKLFGDVQNFTCDALIIATGASARYIGLPSEENYKGRGVSACATCDGFFYRNKPVGVIGGGNTAVEEALYLANIASTVHLIHRRDSFRAEKILIDRLYKKVEEGKIVLHTDRTLDEVLGDNMGVTGLRLANTKTGEKEELKLDGLFVAIGHSPNTEIFQGQLELNNGYIVVKSGLDGNATATSVEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERYLD--

General information: TITO was launched using: RESULT: Template: 5U63.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1865 -208745 -111.93 -673.37 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -111.93 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_5U63.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U63-query.scw PDB file : Tito_Scwrl_5U63.pdb: