Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSFKVALAQFSPHIGNIDSNTQKMIEQANQAKKQDADLIIFPELSVIGYPAEDLLLRPNLNKRMQKAFAQL-SEVKDIVMVFGFVNQTEDGQR-YNSAAVMKDGQVLGVFNKHNLPNYGVFDEKRYFQKGHQH----------------LVF---EYLGHKFGVLICEDIWSINTVKQLSQLNVDTVLV-LNSSPYEVGKPQHRKQTLSELAKQLHLNTVYVNQVGGQD--DLIFDGTSFVSNQNGEIALQAPSF-----------------KEDLYIAEFDR------------------------DTKLYKVVESAPAL--------ETFAEIYQGLVMATRDYVERSGFPGVILGLSGGIDSALTLAIAVDAIGAE-----RVQAVMMPYTYTSQISVEDAAEQARRMGVTFGIAEIHSIVNSFMQTL-YPF-FGNSPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELSVGYCTL-YGDMVGGFAVLKDVYKTIVFELAKYRNSLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDVLDAILYAYIEEDLGQADIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRITSRAFSRERRYPIVNGWTAND 3SEQ Chain:A ((14-539)) ----RVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPKSYLPTYREFYERRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEIAEDMFVPMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMIW-ENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGF------KNNAIKLARALGVTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGE--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3SEQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2281 -41576 -18.23 -97.60 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -18.23 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3SEQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SEQ-query.scw PDB file : Tito_Scwrl_3SEQ.pdb: