Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYTLPIIIKTEKMLKDLKQYY--PLSAYEKKKLFHFLAINLLAEQADSLD-IASNQSL----FFSDIGFYHL-KGVLGEKSVIFINKTLSWLYLHDKLDQKY-L-R-K---NEDETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTIN-K-AD----EFPLDVSFFRENYPAFYCYLIEYINDLTSKRKE--LLQDKFFLFFSYLLLLINHI-PIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVI--E--------AGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE 5WAY Chain:A ((176-493)) ----------PLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEV-----FLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQISVKYQIE-IENKDNLIWHLHNT---AHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDVKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP-YDIIISNFIIPP-IENKRLIYSNNINTVSLIYLLNAMMFIRLDE-----------------------

General information: TITO was launched using: RESULT: Template: 5WAY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 983 6809 6.93 24.85 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 6.93 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_5WAY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5WAY-query.scw PDB file : Tito_Scwrl_5WAY.pdb: