Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYANGGDEMEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFILLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGY---VKTVSEGRLSLVYLTEQGRQFAKEVDGSWEELHQSYKAVLGNEASK---ELAQTMNDATDKLQKK 4G9Y Chain:A ((38-146)) ---------------------------------------------SPQYAVLNALVAEP-GLDQRTVGERVGLDRSTIAEVVSRLGRRGLLDKVRDPQDGRRSLLRLTDEGLRVHRRLGVRIARMNQVFLAPLAADEQAVFFDLIRRVADAAEGL---

General information: TITO was launched using: RESULT: Template: 4G9Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 297 -29257 -98.51 -284.04 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -98.51 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_4G9Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G9Y-query.scw PDB file : Tito_Scwrl_4G9Y.pdb: