Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQIGVLQMRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQPNKEHMDMPSLHSLEHLTAELIRNHAD----YIVDWSPMGCQTGFYLTVLNHESYEDILDVLEATMKDVLEA---TEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA 5E68 Chain:A ((4-149)) -------------LDSFAVDHTRMQAPAVRTAKTMNTPHGDAITVFDLRFCIPNKEVMPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMG-RTGFYMSLIGTPDEQRVADAWKAAMADVLKVQDQNQIPELNVYQCGTYQMHSLSEAQDIARHILER---------

General information: TITO was launched using: RESULT: Template: 5E68.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 -20566 -34.05 -149.03 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -34.05 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_5E68.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E68-query.scw PDB file : Tito_Scwrl_5E68.pdb: