Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQKRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI 1YD0 Chain:A ((1-89)) MKEKIRKKILLAPEEPGVYIFKNKGVPIYIGKAKRLSNRLRSYLNPQTEKVFRIGEE--ADELETIVVMNEREAFILEANLIKKYRPKYNV--------------

General information: TITO was launched using: RESULT: Template: 1YD0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 304 -1167 -3.84 -14.96 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.84 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_1YD0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YD0-query.scw PDB file : Tito_Scwrl_1YD0.pdb: