Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQRDLEKANITILP-FNA---DNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIG-HFIQNYTSIAVTGSHGKTSTTGLLSHVL--SGVRPTSYLIG------------DGTGHGDP--QAEFFSFEACEY-------RRHFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKA--IFAYGDDAYLRKL---KANVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVIAVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQKYPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGAKIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL 3EAG Chain:A ((3-326)) ---AMKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLEALGIDVYEGFDAAQLDEFKADVYVI-GNVAKRGMDVVEAILNLGLPYISGPQWLSENVLHHHWVLGVAGTHGKTTTASMLAWVLEYAGLAP-GFLIGGVPENFGVSARLPQTPRQDPNSQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYLVRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANAD-----GSFDVLLDGKTAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKR-----------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EAG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1545 -27256 -17.64 -93.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -17.64 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_3EAG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EAG-query.scw PDB file : Tito_Scwrl_3EAG.pdb: