Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKFGKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLMETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSAKHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRWAQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK 3RRO Chain:A ((5-250)) --SQFMNLEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSESGAQAISDYLGDNGKGMALNVTNPESIEAVLKAITDEFGGVDILVNNAGITR-DNLLMRMKEEEWSDIMETNLTSIFRLSKAVLRGMMKKRQGRIINVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVTVNTVAPGFIETDMTKALND---EQRTATLAQVPAGRLGDPREIASAVAFLASPEAAYITGETLHVNGGMYM-

General information: TITO was launched using: RESULT: Template: 3RRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1351 -137699 -101.92 -559.75 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -101.92 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_3RRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RRO-query.scw PDB file : Tito_Scwrl_3RRO.pdb: