Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTAEKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQGGYTRILKTEPRRGDGAPMVVLELV 5H1S Chain:P ((19-126)) ----RLSRPADQRRALLRGLTTQLLKHGRIKTTRAKASAMRKYVDKMITLAKEGSLHKRRQALGFI----------YEKQIVHA----LFAEVPERYGDRNGGYTRIIRTLPRRGDNAPMAYIELV

General information: TITO was launched using: RESULT: Template: 5H1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 384 -45519 -118.54 -421.47 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain P : 0.85 3D Compatibility (PKB) : -118.54 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.733

(partial model without unconserved sides chains): PDB file : Tito_5H1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H1S-query.scw PDB file : Tito_Scwrl_5H1S.pdb: