Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCRLRERTRMTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKD-KGKYYTIGGAIQVNEKTEDAVVREVREELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDAPLTMQEDEKSQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE 3I7V Chain:B ((3-131)) -----------------------KEFSAGGVLFKDGEVLLIKTPSNVWSFPKGNIEPGEKPEETAVREVWEETGVKGEILDYIGEIHYWYTLKGERIFKTVKYYLMKYKEGE---PRPSWEVKDAKFFPIKEAKKLLKYK-GDKEIFEKALKLKEK-----

General information: TITO was launched using: RESULT: Template: 3I7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 644 12907 20.04 100.83 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 20.04 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_3I7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3I7V-query.scw PDB file : Tito_Scwrl_3I7V.pdb: