Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRHISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANKAFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDGAGGVLLEASEQ-EHFLAESLNSDGSRS-ECLTYGHSGLHSPFSDQESADSFLKMDGRTVFDFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMMEYGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI 4X0O Chain:E ((3-324)) QCYAEITGWGKCLPPATLSNHDLSTFLDTSDEWIQSRTGIEQRRISH-VNTSDLATVAAQHAIACAGVSVEEIDLIIVATCSPDSLIPNIASRVQQNLGIPSAAAFDLNAA-TGFLYGLETATRLMQASHYRHALVIGAERLSFYLDWTKRDTAVLFGDGAGAVVLSKTEQKVGLQDAQIGCDAQGRDIL-AVPKFGTAMDRFDADNGYWAFDFVGKEIFKRAVRGMGAAAQQVLARSGLSTEEIDVVIPHQANIRIIQTLCDLAGIAQDKAFVNIHRYGNTSAATVPIALCEALEQG--KIKPHDDLLVAAFGAGLTWGAGHIRW

General information: TITO was launched using: RESULT: Template: 4X0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2024 -120028 -59.30 -376.26 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -59.30 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4X0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X0O-query.scw PDB file : Tito_Scwrl_4X0O.pdb: