Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIENASYPLTNCGERTAIFKAISEGQREFSELIVYGQT-EKPISPCGACRQVMAEFFEQDLKVTLVAKDKSTVEMTVGELLPYSFTDLN 1ZAB Chain:A ((14-141)) -HVQRLLLSSREAKKSAYCPYSRFPVGAALLTGDGRIFSGCNIENACYPLGVCAERTAIQKAISEGYKDFRAIAISSDLQEEFISPCGACRQVMREFGTD-WAVYMTKPDGTFVVRTVQELLPASFGPED

General information: TITO was launched using: RESULT: Template: 1ZAB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 647 -39531 -61.10 -311.27 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -61.10 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.661

(partial model without unconserved sides chains): PDB file : Tito_1ZAB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZAB-query.scw PDB file : Tito_Scwrl_1ZAB.pdb: