Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSDIENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGD-FKKVMTDDQTYETRTVIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLTRFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGEN-RVESVVFENVKTGQVTEQAFGGVFIYVGLDPLSDFVKE-LNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLKDLRQVTTAVGDGAIAGQEAYKFITEHS 5MIT Chain:A ((24-325)) KYDVVIIGSGPAGMTAAMYTARSEMKTLLLERGVPGGQMNNTAEIENYPGYETIMGPELSMKMAEPLEGLGVENAYGFVTGIEDHGDYKKIIT-EDDEFITKSIIIATGANHRKLEIPGEEEYGARGVSYCAVCDGAFFRNQEILVIGGGDSAVEEALYLTRFGQSVTIMHRRDKLRAQEIIQQRAFKEEKINFIWDSVPMEIKGDDKKIQSVVYKNVKTGEVTEKAFGGIFIYVGLDPVAEFVSDLGIT-DEAGWIITDDHMRTNIPGIFAVGDVRQKDFRQITTAVGDGAQAAQEAYKFVVE--

General information: TITO was launched using: RESULT: Template: 5MIT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1819 -43788 -24.07 -146.45 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -24.07 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_5MIT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MIT-query.scw PDB file : Tito_Scwrl_5MIT.pdb: