Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLRIEKNNGIATVYLNRPDKRNAMSFALLKELVTTAKAIQKDRDIRCVILTGEAQVFSAGIDLSDLNNPKNSAFAIWELVKPGQSLFQKAFLIWQNLPVPVIAALEGYCFGAGMQLALAADIRIAHPDTKMSIMESRWGLVPD-MGLTRSLKGLIGVDLAKELTLTARVLDGNYAKDIGLVTHLSE--NPLEKANAIASEMLQRSPDALTAAKRVLDAMEHQPE-KSLRLEKIWQLKLLLGKNSKLARKKDK---HPEVKFLPRQYR 3G64 Chain:C ((19-277)) ---LRVEITDGVATVTLARPDKLNALTFEAYADLRDLLAELSRRRAVRALVLAGEGRGFCSGGDVDEIIGATLSMDTARL-LDFNRMTGQV-VRAVRECPFPVIAALHGVAAGAGAVLALAADFRVADPSTRFAFLFTRVGLSGGDMGAAYLLPRVVGLGHATRLLMLGDTVRAPEAERIGLISELTEEGRADEAARTLARRLADGPALAHAQTKALLTAELDMPLAAAVELDASTQALLMTGEDYAEFHAAFTEKRPPKWQGR-----

General information: TITO was launched using: RESULT: Template: 3G64.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1220 -104703 -85.82 -415.49 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -85.82 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_3G64.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G64-query.scw PDB file : Tito_Scwrl_3G64.pdb: