Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPKEHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAA--GMNIISNCEEVAGQTVFHTHVHLVPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA 3N1S Chain:N ((4-105)) --ETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIPNILIPTVNDVSAEH-EQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHMHLL---------------------------------

General information: TITO was launched using: RESULT: Template: 3N1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 389 -71184 -182.99 -711.84 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain N : 0.74 3D Compatibility (PKB) : -182.99 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3N1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N1S-query.scw PDB file : Tito_Scwrl_3N1S.pdb: