Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQQAKSRKPQQPEYIFQVETLSHEGRGIAHYGSHPDHPADKHGKKVFIRYALPGETVKAQITHEAKRLEEAEMVALLAEPSANRVEAVCPHYGICGGCSMQHIHPDEQIRLKQNVLQSHLQHFAGIQPEQWLEPIRSLQSDYRRRARIGVRYLPKQDRLILGFREHHSNRLTSIHTCSVLDKKLSDSLPELRNLLQSLKGKVHIGHVELAKGDHETSLLVRHIEKLNNADVNQLRQFALHKGWQLYLQPKGPESLRRIDEEQGAMRLHYALNAFDVNFAFSPLDFTQVNATVNEQMVQLACELLQLQQGERVLDLFCGLGNFSLPLARCVGAKGVIVGVEASEEMVQRATDNVKRNNLVQASFFSQDLTKDFSHHSWANQGFDALLIDPPRAGAYEI--IQYVPN-FGAKRIVYVSCNPATLARD-AGVLVQ-HGYQLKK------AAVMDMFTHTEHVESIALFEKIQEIND 1WY7 Chain:D ((48-195)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IEGKVVADLGAGTGVLSYGALLLG-A-KEVICVEVDKEAVDVLIENLGEFK-GKFKVFIGDVSEF---NS----RVDIVIMNPPFGSQRKHADRPFLLKAFEISDVVYSIHLAKPEVRRFIEKFSWEHGFVVTHRLTTKIEIPLQFFFHRKKLERITV---------

General information: TITO was launched using: RESULT: Template: 1WY7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 596 -58687 -98.47 -428.37 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : -98.47 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_1WY7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WY7-query.scw PDB file : Tito_Scwrl_1WY7.pdb: