Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKMKSPILRLRRLRRTKAIRGLLCENNFSVEDLVQPIFIEESITQPIPISTMPGIYRLPEQ-DIEQEVKALYALGIRCVMPFGISHH--KDSMGSDTWSDTGLLVRMIKAIKSSCP-EMIVTPDICFCEYTTHGHCGVMIHD----QISNDLTVENLIKQSLAAAKAGADMLAPSGMMDGQVGAIREALDQAGYHNVLIMAHAIKFASALYGPFRVAVDS-NLVGDRHSYQLDYANTRQALREAALDESEGADLLIVKPGMFYLDILSQLRAQT-KLPLAAYQVGGEYAAIKFAAIAKALDERKTVIESITAFKRAGADVIITYFAKDIAIWHNEF 5LZL Chain:G ((3-334)) ---VQFPTTRPRRLRASKIIRDAVAETQIDAGDFIYPLFVKPGG-EREPIGPMPGIYRWPVGRELINHVEEALSLGINKFILFGVLPDELKNPEGTGGYDPEGVVPRAIRLIKEIFGDRVLVFADVCLCEYTDHGHCGVVKEKRDRWYVDNDETIKLYAKEAVVYAEAGADFVAPSGMMDGQVREIRRALDAHGFEEVGIMAYSAKYASAFYGPFRVAAASAPKFGDRRTYQMDPRNAYEALKEVAMDLEEGADIVMVKPALAYLDVIRLVKQHFPWVPLAAYNVSGEYSLVKAAATAGYVDERTITLEILTAIKRAGADLILTYHALEAAKWIKE-

General information: TITO was launched using: RESULT: Template: 5LZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1732 -71953 -41.54 -223.46 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain G : 0.83 3D Compatibility (PKB) : -41.54 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_5LZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LZL-query.scw PDB file : Tito_Scwrl_5LZL.pdb: